HMS Hepline kit

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Hi!

For those still using the HMS Plus "HEPline kit", can someone explain how to correlate the U/mL on the supplied tube to the Heparin Concentration in mg/kg that is resulted by the HMS? 

If the 1 U/mL was filled to the 5mL line with healthy donor blood and produced a result of 0.6 mg/kg, how do I know if that is acceptable?

Thank you for any help!

3 Replies

Here's what we have in our policy: ยท         
The Hepline kit consists of 5 tubes containing increasing concentrations of heparin.  The tubes are designed to give final concentrations of 1, 2, 3, 4, 5 units/mL when freshly drawn whole blood is added; 5 mLs blood into each predosed tube.  Results for the concentration needs to fall within the corresponding cartridge test range.  For example, tube 2 which contains a concentration of 2 units/mL needs to fall within the Tan cartridge range of 1.5-3.0.

Ok that makes sense. I was unable to find a Medtronic source to verify.
We only use the red and silver cartridges. We ran the 1 u/mL on the red, and it worked for the cartridge, and used the 2 and 3 u/mL on the silver, which both produced a 2 mg/kg result. 
Do you think I should I only use the 1 and 3 tubes? Trying to cover the alternative proficiency requirement. 

Ahh yes if you don't use all the color cartridges, then I think you would pick which tubes coordinate with the concentrations. 

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