Wet Prep processes
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For any facility that is performing wet preps:
- Is anyone utilizing a stain to differentiate WBCs? If so, what stain do you use?
- Has anyone had a clinician wanting to know if eosinophils are present?
- Has anyone had a clinician wanting a semi-quantitative estimate of the number of WBCs and squamous epithelial cells? If so, how do you quantitate?
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We use phase contrast microscopy, no staining. We do not report eosinophils.
We report the following:
Epithelial cells
WBCs
Clue cells
Bacteria
Yeast
T. vaginalis
Fungal hyphal elements
We report as:
None seen, NS
Rare, RA (0-1/HPF)
Few, FE (2-5/HPF)
Moderate, MOD (5-10/HPF)
Numerous, NUM (>10/HPF)
We use a similar reporting and quantitation system, but not at the point-of-care. Regarding staining, I think that the addition of a differential stain takes the test out of the approved list of nine PPMP tests, and therefore ineligible for POC. A quick google search shows that there may be some clinical value to identifying eosinophils in a wet mount (allergic response to spermicides or douches), but I would argue that such a test is best suited to a laboratory setting.
Forgot to mention, we don't perform Wet Prep point of care. Only done in the main laboratory.
I should have clarified that we only perform Wet Preps in the hospital lab as well. We do not allow any PPMP in our organization for good reason. We have so many projects going on right now, I hate to bring on another process change/validation and associated training and competency to go with it if it won't bring value or improve patient care.