MetHb% Avox 4000
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For those that use the Avox 4000, what is your facilities cutoff for reportable range on your MetHb% for your AMR. The Cal ver materials from RNA medical get us to reach all the instrument reportable ranges except for this analyte.
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Hello Everyone: What CalVer material do you use for AMR verification of MetHb on the Co-Oximeter?
Dianne, did you find a different Calibration Verification material to use for MetHb?
I tested RNA medical CVC-223 lot# 922335 on the Avox 4000 and ran into the same problem. All five solutions have very similar ranges and therefore they do not create a linear relationship for MetHb. The reportable range according to the operator's manual is %MetHb 0 to 85%.
CVC-223 CalVer Ranges:
Does anyone use a different product other than RNA medical?
Try reaching out to IL (Instrumentation Laboratories). Since acquiring this instrument I know they are marketing their own QC product for the AVOX but I'm not sure about cal ver. I haven't had a chance to try out any of these new products yet.
I have reached out to IL regarding this and was told that no-one had Cal/Vers for MethHGB. This was a couple years ago however.
Not sure if it is compatible, but Radiometer has one, VK5, that goes from 0.0 to 30.
We are in the process of installing the GEM 5000 (previously used Avox 4000). We were told by IL that the Met Hgb is indirectly measured, therefore it doesn't require linearity and AMR verification. The tHGB is the analyte directly measured so it is the only co-ox analyte that requires linearity and AMR. (The AVOX did not have anything available either.)
"Met Hgb is indirectly measured, therefore it doesn't require linearity and AMR verification. The tHGB is the analyte directly measured"
Can't say I follow the logic in this. The sample is placed in a chamber that measures the different wavelengths absorbed by the sample. How does one say the sample is directly measured for HGB and indirectly for MET-HGB?
The above statement is also in opposition to manufactures Avoximeter 4000 Operators Manual, see the bottom of page 6:
"The AVOXimeter 4000 measures oxygenated hemoglobin (O2Hb), reduced hemoglobin (HHb), methemoglobin (MetHb), and carboxyhemoglobin (COHb) directly, using novel optics and multiple wavelengths. This reduces interference from dyshemoglobins and other interfering substances such as fetal hemoglobin and bilirubin and minimizes the effects of hemolysis."